Relationship between freezing rate, ultrastructure and recovery in a human diploid cell line.

Evaluation of Embryos Derived from in vitro Fertilized Oocytes Reconstructed by Meiosis-II Chromosome Transplantation from Aged Mice to Ooplasms of Young Mice

Background To assess embryos derived by the transfer of meiosis-II chromosomes (M-II-t) from aged mice oocytes into ooplasms from younger mice to overcome the problem of age-related decline in female fertility. MaterialsAndMethods The developmental capacity karyotype and ultrastructure of reconstructed oocytes derived from meiosis-II chromosome transplantation from aged mice into the ooplasms o...

The Isolation and Characterization a Human Diploid Cell Strain and Its Use in Production of Measles Vaccine

Effect of Helium-Neon Laser Irradiation on Motility and Ultrastructure of Fresh and Frozen-Thawed Human Sperm

Purpose: Semen freezing is an important technique in human infertility treatment in many different patients but it damages sperms. Low-power lasers are physical agents that have biostimulatory effects. In this study, low-power helium-neon laser's (16J/cm2, one time irradiation) effect on motility and ultrastructure of fresh and frozen-thawed human sperm were studied. Materials and Methods: Thi...

Mesenchymal Stem/Stromal-Like Cells from Diploid and Triploid Human Embryonic Stem Cells Display Different Gene Expression Profiles

Background: Human ESCs-MSCs open a new insight into future cell therapy applications, due to their unique characteristics, including immunomodulatory features, proliferation, and differentiation. Methods: Herein, hESCs-MSCs were characterized by IF technique with CD105 and FIBRONECTIN as markers and FIBRONECTIN, VIMENTIN, CD10, CD105, and CD14 genes using RT-PCR technique. FACS was performed fo...

Polarized and Non-Poarized Human Oviduct Epithelial Cell Ultrastructure in Vitro

Purpose: This study designed to examine polarized culture of epithelial cells from human ovidutc and their ultrastracture under polarizing condition. Materials and Methods: The human oviduct was obtained from patients having undergone total hysterectomy and epithelial cells were isolated using collagenase type I. The epithelial cells were either cultured on ECM (Extracellular matrix) Gel coate...